Functional clustering of T cell and other immune cell sub-phenotypes across plasticity and individualities of immune-mediated diseases.

Project start
Institution: Institute for Medical Microbiology and Hygiene, Philipps-Universität Marburg
Applicant: Prof. Dr. Michael Lohoff
EKFS funding line: First and Second Applications
Comparison of human lymphocytes from four donors by CyTOF®.

With the support of the Else Kröner-Fresenius-Stiftung, we are unifying efforts to better describe immune cells in healthy individuals and also in people suffering from chronic infection, allergy, asthma, and inflammatory bowel disease. Our project focuses on lymphocytes, specifically the ones called CD4 T-lymphocytes. For the first time, CD4 T-lymphocytes appearing in these diseases over time are coordinately analysed in a deeper and extended manner. Together with our collaborators at the universities of Marburg, Mainz, and Würzburg, we are using a new technology called CyTOF® (Time of Flight Mass Cytometry) to reach this goal. Blood and affected tissue samples from our donors gets analysed in our core facility in Berlin where we take advantage of the most experienced CyTOF® facility in Germany. 

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