Non-contact manipulation of cells opens up new possibilities in tissue engineering. Through superparamagnetic iron oxide nanoparticles (SPIONs), cells can be made magnetically controllable. When comparing five different SPION systems, the magnetic steerability of the cells depended on the amount of cellularly ingested particles.
Using appropriate magnetic fields, we were able to seed the cells into cell culture dishes in a targeted manner. Magnetic loading and sedimentation accelerated the formation of multicellular spheroids from many thousands of cells. Simultaneous use of non-loaded and SPION-loaded cells resulted in polarity in the cellular spheroids as the SPION-loaded cells aligned on the side of the magnetic field. In the future, remote control of different "magnetized" cell types could be used to build multilayered tissues without the need for a scaffold structure.
Kappes M, Friedrich RP, Pfister F, Huber C, Friedrich RP, Stein R, Braun C, Band J, Schreiber E, Alexiou C, Janko C.
Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture. Adv. Funct. Mater., 2022, 2203672
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